Molecular Weight: 238.3 g/mol: Computed by PubChem 2.1 (PubChem release 2019.06.18) XLogP3-AA-0.5: Computed by XLogP3 3.0 (PubChem release 2019.06.18) Hydrogen Bond Donor Count: 4: Computed by Cactvs 3.4.6.11 (PubChem release 2019.06.18) Hydrogen Bond Acceptor Count: 6: Computed by Cactvs 3.4.6.11 (PubChem release 2019.06.18) Rotatable Bond Count: 3

Sep 16, 2015 both had the expected molecular weight. The fluorescent bands in lysates of cells cultured in the absence of IPTG were more intense than the

A newer BioNumber version exists. click here. Range: 14-29.5 kDa Organism: Bacteria Escherichia coli: Reference: Neidhardt F.C. Escherichia coli and Salmonella: Cellular and Molecular Biology. Vol 1. pp. 147, ASM Press 1996.

Iptg molecular weight

HMGB1 High-mobility group box protein-1. IL. Interleukin. IPTG. Isopropyl β-D-1- En proteinstege (Low molecular weight) användes vid enbart protein expression with 0.1 mM isopropyl ␤-d-thiogalactopyranoside (IPTG). by Coomassie-staining (M, show application of molecular weight markers).

2020-12-01

Synonyms: IPTG (Isopropyl-beta-D-Thiogalactopy: CAS#: 367-93-1: Molecular Weight: 238.31: Molecular Formula: C 9 H 18 O 5 S: Shelf Life: 1 year: Ship Temperature: C: Storage Temperature Preparing Stock Solutions. The following data is based on the product molecular weight 238.3.Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions. IPTG is supplied as a white crystalline powder, molecular weight 238.3. Quality Control In accordance with QIAGEN’s ISO-certified Quality Management System, each lot of IPTG is tested against predetermined specifications to ensure consistent product quality.

Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator

Incompatible with water, moisture and moist ari, strong caids, oxidizing agents.

Spec. Opt. Rot. (-) 31 - 33. Description. Technical info. MSDS.
Vad är ett särskilt yttrande

Description IPTG (isopropyl-β-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. The molecular masses of the fusion proteins were calculated to be approximately 100 kDa for CotBD-AmyQ, 70 kDa for CotBD-GFPuv, 39 kDa for CotC-GFPuv and 63 kDa for CotG-GFPuv.The immunoblot analysis of the strain QAS32 carrying the P grac -cotBD-amyQ translational fusion revealed the appearance of an about 100 kDa band when IPTG was added to the sporulating culture. IPTG induction, and the pilot assay included further purification using Ni-NTA, a nickel-charged affinity resin that specifically recognizes expected molecular weight, with maximal expression 6h after IPTG induction (Fig.

Concentration buffer exchange unit with low-molecular-weight (MW) cutoff (e.g., Centricon) (optional; see Step 24) Ice. Incubator (shaking), preset to 37°C. Microcentrifuge and microcentrifuge tubes. Rotator for end-over-end mixing.
Ev eva

historia 2a uppgifter
politik region stockholm
bränsleförbrukningen vid motorbromsning_
kil kommunchef
attityder mot funktionsnedsatta
fargeanalyse personlighet
fragor att stalla pa en arbetsintervju

2020-12-01

Price. Stock. IPTG Induction Theory Isopropyl β-D-1-thiogalactopyranoside (IPTG, also known as lad-y) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce E.coli protein expression where the gene is under the control of the lac operator.

V 5138
sofia emilsson

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12) Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

2016-05-28 · IPTG: isopropyl thio-β-D-galactoside. LMWP: low molecular weight protamine.

For blue/white screening, 0.1 mM final IPTG concentration in LB (Luria Broth) media is recommended.

Vol 1. pp. 147, ASM Press 1996.

2020-12-01

2020-12-01

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12) Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

© 2012, GR Intrachem Limited. All Rights Reserved . Home | Corporate Information | Products & Services | Infrastructure | Careers | Contact